A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors

Authors

Jack R. Bateman, Harvard Medical School
C. Ting Wu, Harvard Medical School

Document Type

Article

Publication Date

11-1-2008

Abstract

Here we describe a simple method for generating donor vectors suitable for targeted transgenesis via recombinase-mediated cassette exchange (RMCE) using the ΦC31 integrase. This PCR-based strategy employs small attB "tails" on the primers used to amplify a sequence of interest, permitting the rapid creation of transgenes for in vivo analysis. Copyright © 2008 by the Genetics Society of America.

Recommended Citation

Bateman, Jack R. and Wu, C. Ting, "A simple polymerase chain reaction-based method for the construction of recombinase-mediated cassette exchange donor vectors" (2008). Biology Faculty Publications. 23.
https://digitalcommons.bowdoin.edu/biology-faculty-publications/23