Year of Graduation
2022
Level of Access
Open Access Thesis
Embargo Period
5-19-2023
Department or Program
Biochemistry
First Advisor
Bruce Kohorn
Abstract
Plant cell growth and development relies on proper cellular adhesion. As the extracellular matrix serves as the area of connection between two cells, its synthesis and maintenance are essential for cellular adhesion. The middle lamella region, the layer of the extracellular matrix between two adjacent cell walls, is diffuse with the polysaccharide pectin due to its delivery by Golgi vesicles early during cell division. A Ruthenium Red screen for cellular adhesion mutants identified the family of 5 ELMO proteins that are critical for proper cellular adhesion. To further our understanding of plant cellular adhesion and pathways of pectin synthesis and modification, this work investigates ELMO5. Plants homozygous for a T-DNA insertion in ELMO5 and a new deletion mutant allele generated using CRSPR do not have a cellular adhesion phenotype, suggesting it is either not critical for cellular adhesion or is redundant with another gene. Redundancy within the ELMO family is identified through the analysis of double mutants of elmo5 and each of the other four elmo genes. Both elmo1-/- elmo5-/-and elmo4-/- elmo5-/-mutants have a visibly worse cellular adhesion defect phenotype, suggesting partial redundancy through the ELMO family. The mutants are also rescued by growth on agar, pointing to the importance of turgor pressure and osmotic potential in modulating cellular adhesion. Both ELMO4 and ELMO5 were found to localize to the Golgi using a GFP fusion, consistent with a role for ELMOs as scaffold for pectin biosynthesis.