The twin spot generator for differential Drosophila lineage analysis

Authors

Ruth Griffin, CNRS Centre National de la Recherche Scientifique
Anne Sustar, University of Washington
Marianne Bonvin, University of Washington
Richard Binari, Harvard Medical School
Alberto del Valle Rodriguez, New York University
Amber M. Hohl, Harvard Medical School
Jack R. Bateman, Harvard Medical School
Christians Villalta, Harvard Medical School
Elleard Heffern, Harvard Medical School
Didier Grunwald, Inserm
Chris Bakal, Harvard Medical School
Claude Desplan, New York University
Gerold Schubiger, University of Washington
C. Ting Wu, Harvard Medical School
Norbert Perrimon, Harvard Medical School

Document Type

Article

Publication Date

7-28-2009

Abstract

In Drosophila melanogaster, widely used mitotic recombination-based strategies generate mosaic flies with positive readout for only one daughter cell after division. To differentially label both daughter cells, we developed the twin spot generator (TSG) technique, which through mitotic recombination generates green and red twin spots that are detectable after the first cell division as single cells. We propose wide applications of TSG to lineage and genetic mosaic studies.

Recommended Citation

Griffin, Ruth; Sustar, Anne; Bonvin, Marianne; Binari, Richard; del Valle Rodriguez, Alberto; Hohl, Amber M.; Bateman, Jack R.; Villalta, Christians; Heffern, Elleard; Grunwald, Didier; Bakal, Chris; Desplan, Claude; Schubiger, Gerold; Wu, C. Ting; and Perrimon, Norbert, "The twin spot generator for differential Drosophila lineage analysis" (2009). Biology Faculty Publications. 21.
https://digitalcommons.bowdoin.edu/biology-faculty-publications/21